Movie 6.

Ectopic microvilli are enriched in CD9. Confocal z-stack of an MII oocyte treated with CK-666 (100 μM) for 3h, then fixed and labeled for F-actin (Alexa Fluor 568-phalloidin, red) and CD9 (green). Chromosomes are labeled with TO-PRO-3 (magenta). The red halo at the start of the series is due to the F-actin signal in the first polar body. Confocal section thickness is 1 μm.