Movie 5.

Actin polymerization is important for pkcA-GFP containing vesicular movement. To ascertain if actin polymerization is important for pkcA-GFP movement, Ax2/act15::pkcA-GFP cells were treated with 10 μM Latrunculin B and pkcA-GFP localization was visualized. Lat B treated cells round up and although the membrane localization of pkcAGFP was intact, pkcA-GFP containing vesicles clutter together and showed no further movement. Thus, for vesicular pkcA-GFP localization actin polymerization is important.