Dynamics of single GFP-ELKS molecules in an isolated mouse pancreatic islet. An example TIRFM acquisition of single GFP-ELKS molecules (top left) and the corresponding overlay of trajectories (bottom left). Right panels illustrate the temporal median filtering method, the splitting of the acquisition into fast (top) and slow (bottom) components.
Ivar Noordstra, Cyntha M. van den Berg, Fransje W. J. Boot, Eugene A. Katrukha, Ka Lou Yu, Roderick P. Tas, Sybren Portegies, Bastiaan J. Viergever, Esther de Graaff, Casper C. Hoogenraad, Eelco J. P. de Koning, Françoise Carlotti, Lukas C. Kapitein, and Anna Akhmanova
J Cell Sci
2022. 135:None-None; doi: 10.1242/jcs.259430