Movie S5

mRNA of Cox7c-MTS STOP MS2 visualized with MCP-GFP construct and displays low frequency of co-localization with mitochondria in axons. Live-cell imaging of mRNA particles (green) together with mitotracker-marked mitochondria (red) in a motor neuron axon. The red arrow indicates Cox7c-MTS-STOP mRNA particles co-localizes with static mitochondria. Note that moving mRNA puncta are not colocalized with mitochondria. Movie speed is 10 frames per second, at 3 seconds per frame.