Movie S1
Dual-color SMCT identifies FHC association in live cell plasma membranes. GFP-Kb dimer formation was probed by co-locomotion analysis. Individual GFP-Kb molecules labeled with Rho11 (magenta) and Dy647 (green) in STF1 cells were imaged by TIRF microscopy (scale bar, 2.5 μm; frame rate, 10 Hz; playback speed, 1/3 real time). Movies were acquired in the absence of peptide (FHCs, left), in the presence of peptide (HC/b2m/peptide complexes; center) and in the presence of peptide and a crosslinker consisting of a dimeric anti-GFP nanobody (right). Localized molecules in each channel are encircled, co-locomoting molecules are highlighted by white diamonds.
Dissociation of β2m from MHC class I triggers formation of noncovalent transient heavy chain dimers
J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.259498