Movie S2

SMCT demonstrates transient association of FHCs. Individual GFP-Kb molecules labeled with Rho11 (magenta) and Dy647 (green) imaged by TIRF microscopy (scale bar, 450 nm; frame rate, 10 Hz; playback, 1/3 real time). selected image section showing SMCT of an individual FHC complex (i.e., in the absence of peptide). Localized molecules in each channel are encircled, co-locomoting molecules are highlighted by white diamonds, with the dissociation occurring between 4.03 s and 4.06 s.

Dissociation of β2m from MHC class I triggers formation of noncovalent transient heavy chain dimers

Cindy Dirscherl, Sara Löchte, Zeynep Hein, Janine-Denise Kopicki, Antonia Regina Harders, Noemi Linden, Andreas Karner, Johannes Preiner, Julian Weghuber, Maria Garcia-Alai, Charlotte Uetrecht, Martin Zacharias, Jacob Piehler, Peter Lanzerstorfer, and Sebastian Springer

J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.259498