Movie S5

SMCT demonstrates transient association of FHCs. Individual GFP-K^b molecules labeled with Rho11 (magenta) and Dy647 (green) imaged by TIRF microscopy (scale bar, 450 nm; frame rate, 10 Hz; playback, 1/3 real time). selected image section showing SMCT of an individual FHC complex (i.e., in the absence of peptide). Localized molecules in each channel are encircled, co-locomoting molecules are highlighted by white diamonds, with the dissociation occurring between 4.03 s and 4.06 s.