Movie 3.

MDA-MB-231 control and STX7KO clonal cells (STX7KO) were transfected with pHluorinMT1-MMP. Cells were plated on a gelatin-coated imaging dish, and videos were captured using a TIRF microscope (Fig. 3A). Scale bar 10 μm. Exposure time, TIRF Green: 600ms (Control); 800ms (STX7KO).