Movie 4.

MDA-MB-231 cells were transfected with SHC002 and shSTX4. Depleted cells were transfected with pHluorinMT1-MMP. Cells were plated on a gelatin-coated imaging dish, and videos were captured using a TIRF microscope (Fig. 3A). Scale bar 10 μm. Exposure time, TIRF Green: 400ms (SHC002); 500ms (shSTX4).