Movie 8.

MDA-MB-231 STX7KO clonal cells (STX7KO) were transfected with SHC002 and shSTX4. After 48h of depletion, cells were transfected with pHluorinMT1-MMP. Cells were plated on a gelatin-coated imaging dish, and, videos were captured using a TIRF microscope (Fig. 4I). Scale bar 10 μm. Exposure time, TIRF Green: 400ms.