Movie 3

Time-lapse movie of embryo (stage 15) expressing EGFP-Stac (green) and palm-mKate2 (magenta) in tracheal cells. A single confocal section containing an EGFP-Stac-positive EV is shown. Note that EGFP-Stac-EVs are immobile inside the tracheal lumen, but start to move and subsequently disappear rapidly during luminal liquid clearance (beginning at t=240 minutes).