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Movie 2.

Time-lapse imaging of type A HeLa cells expressing DsRed-CRIPTC3Y. Cells transfected with DsRed tagged CRIPTC3Y were photographed as described for CRIPTWT. During 24-hour recording, three types A cells in a representative field were captured, and all three cells were recoded to progress into metaphase within 15 minutes after the onset of mitosis, while their spindle halves were continuously moving back and forth over 8 hours till the end of the recording.

Mitotic spindle disassembly in human cells relies on CRIPT having hierarchical redox signals

Kehan Xu, Chunxue Wang, Kari Keinänen, Hong Li, and Chunlin Cai

J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.259657