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Movie 5.

Time-lapse imaging of type B HeLa cell expressing DsRed-CRIPTC3Y. The mitotic cell progressed into metaphase less than 15 min after the mitosis onset at around 12:00. The spindles were formed normally and were seen to move back and forth for 2 hours. At around 14:00, the spindle halves were completely separated, then rapidly gathered to form a messy fiber-like structure that exists till the end of the recording.

Mitotic spindle disassembly in human cells relies on CRIPT having hierarchical redox signals

Kehan Xu, Chunxue Wang, Kari Keinänen, Hong Li, and Chunlin Cai

J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.259657