Movie 5.

Nuclear F-actin dynamics in Lamin A knockdown HeLa cells expressing actinWT-NLS and nAC-GFP. G1-synchronized HeLa cells were seeded on glass-bottom dishes, co-transfected with siRNA against Lamin A and expression plasmids for actinWTNLS and nAC-GFP, and imaged live by confocal microscopy 24 hours after transfection. A 20-hour timelapse was acquired, imaging every 4 hours. Nuclei were cropped at each time point to account for cell migration. The yellow arrowhead in the first frame indicates a site of actin accumulation at the nuclear rim, and frame 3 corresponds to mitosis.