Movie 7.

Nuclear F-actin dynamics in Lamin A knockdown HeLa cells expressing actinWT-NLS and nAC-GFP. G1-synchronized HeLa cells were seeded on glass-bottom dishes, co-transfected with siRNA against Lamin A and expression plasmids for actinWTNLS and nAC-GFP, and imaged live by confocal microscopy 20 hours after transfection. A 1.5-hour timelapse was acquired, imaging every 10 minutes. The yellow arrowhead in the first frame indicates a site of actin accumulation at the nuclear rim, and only interphase is shown.