Movie 1.

Live-cell microscopy showing fluorescence recovery after photobleaching of WT D. 83 discoideum cells. WT D. discoideum cells were incubated in SIH without polyP, and fluorescence recovery after photobleaching was monitored over time. Fluorescence images were collected every 0.5 seconds for 59 seconds using a 60× water-immersion objective. Bar is 10 μm. Indicated time after bleaching is in minutes:seconds.