Movie 2.

Fn1 fibrillogenesis imaged by TIRF microscopy. Fn1^mEGFP MEFs were transiently transfected with mCardinal-Lifeact, plated on gelatin-coated glass coverslips, and imaged 48 hours later. Filming was done every 2 min for 30 min using TIRF and 100x objective, N.A. 1.49. The first set shows the Fn1-mEGFP channel. Yellow arrows point to centripetally-moving Fn1 nanodomains organized into an elongating linear fibril. The second set is an overlay between Fn1-mEGFP and mCardinal-Lifeact.