Movie 3.

2% DOC dissolved cytoplasm and nucleus in under 13 min leaving cell-free Fn1 fibrils. MEFs expressing Fn1-mNeonGreen were plated on glass-bottom slides without coating and labeled with SiRActin (magenta) to visualize Factin, and Hoechst (blue) to visualize DNA. Time-lapse was recorded every 54 sec immediately following the addition of the DOC solution pH 8.01 to live cells. The presence of 2% DOC dissolves actin cytoskeleton and nuclei and leaves cellfree Fn1 ECM fibrils (green). Fn1 fibrils collapse following the dissolution of the actin cytoskeleton due to the loss of tension.