Movie 5.

Fibrillogenesis of ectopic Fn1. 3-well culture insert was placed in the middle of the 35 mm glass-bottom dish and 0.8 x 10⁶ Fn1-tdTomato-expressing MEFs were plated surrounding the inserts. 24 hours later, Fn1-mEGFPexpressing MEFs were plated inside the inserts on glass without coating for 5 hours. Confocal live imaging of areas containing Fn1-mEGFP-expressing MEFs was performed using Plan Fluor 40x Oil (NA 1.3). Positions containing Fn1-mEGFP-expressing MEFs were imaged at 17–18 min intervals for ∼16 hours. This movie contains maximum intensity projections composed of forty-three confocal slices at 0.5 μm thickness, the pinhole was set to 1 Airy unit. The first still panel in this movie is a montage of the plate to show Fn1-tdTomato and Fn1-mEGFP-expressing cells prior to the start of the time-lapse.