Movie 7.

FUD interferes with the linking of centripetally moving Fn1 nanodomains into fibrils. Fn1^mEGFP/+ MEFs were plated on glass in 8-well Ibidi chambers for 4 hours. Medium containing FUD peptide was then added and cells were filmed every 3 min for about 15 hours, as described in Methods. The movie begins approximately 30 min after the FUDcontaining medium was added, the time it takes to set up a time-lapse recording. Note the dismantling of the remaining pre-existing fibrils at the beginning of the movie. Yellow and red arrows point to the cell periphery. Note the presence of centripetally moving Fn1-mEGFP “beads” and the scarcity of Fn1 fibrils for the majority of the duration of the movie.