Movie 3.

Movies of GFP-GtaC^c-s translocation in receptor and G protein mutant strains. Cells stimulated with 1 μM folate (S3A-C) or 10 nM cAMP (S3D-F). Time-lapse video with images every 30 sec. Stimulant added at time 0 min (image 2, contains red dot). Order of movies: 3A: far1^- cells 3B: gα4^- cells 3C: gα5^- cells 3D: far1^- cells 3E: gα4^- cells 3F: gα5^- cells