Movie 4.

Movies of GFP-GtaC^c-s mutants with altered phosphorylation sites. Wild-type cells expressing translocation in folate strains stimulated with 1μM folate. Timelapse video with images every 30 sec. Stimulant added at time 0 min (image 2, contains red dot). Order of movies: 4A: GtaC^S357A (p911) 4B: GtaC^T492A (p913) 4C: GtaC^S380A (p926) 4D: GtaC^S386A (p925) 4E: GtaC^S357A,T492A (p937) 4F: GtaC^S380A,S386A (p929) 4G: GtaC^{S357A,S380A,S386A} (p931) 4H: GtaC^{S380A,S386A,T492A} (p932) 4I: GtaC^{S357A,S380A,S386A,T492A} (p935)