Movie 4.

IMV motility on GMPCPP microtubules (also see Fig. 3C) Example of IMV (magenta) labelled with RFP-A3 (indicated by the white arrowheads) moving on GMPCPP-stabilised microtubules (blue) in vitro in the presence of 2 mM ATP (left panel) but not AMPPNP (right panel). The time in minutes:seconds is indicated, and the scale bar = 5 μm. Images were taken every second. Video plays at 10 frames per second.