Movie 5.

IEV motility on GMPCPP microtubules (also see Fig. 3C) Example of IEV (magenta) labelled with RFP-A3 and A36-YdF-YFP (green), as indicated by the white arrowheads, moving on GMPCPP-stabilised microtubules (blue) in vitro in the presence of 2 mM ATP (left panel) but notAMPPNP (right panel). The time in minutes:seconds is indicated, and the scale bar = 5 μm. Images were taken every second. Video plays at 10 frames per second.