Movie 6.

IEV move towards the plus end of microtubules (also see Fig. 3G) Example of IEV labelled with RFP-A3 (magenta) and A36-YdF-YFP (green), as indicated by the white arrowheads, translocating towards the bright microtubule plus-end (blue) in vitro in the presence of 2 mM ATP. The time in minutes:seconds is indicated, and the scale bar = 10 μm. Images were taken every second. Video plays at 20 frames per second.