Movie 1.

Representative video of fluorescent protein recruitment during Chlamydia invasion Cos7 cells were transfected with plasmids expressing various GFP-fusion proteins for 24 hours prior to infection with CMTPX-stained C. trachomatis EBs (MOI=20). Bacterial adhesion and entry was monitored by live-cell imaging using a spinning disk confocal microscope (Nikon CSU-W1 or Leica SD6000 AF), obtaining images every 20 seconds for 30 minutes to identify sites exhibiting protein recruitment. Images were assembled into videos in ImageJ and annotated to highlight recruitment events. The representative video shown here depicts GFP-actin recruitment at sites where CMTPX-Chlamydia contacts the host cell (arrows). Similar videos were assembled for all proteins monitored throughout the study, each yielding recruitment events that exhibit tight localization around fluorescent Chlamydia, enabling straightforward quantification of recruitment intensity and duration (Figs. S1-3).