Movie 1.

Visualisation of canonical Wnt pathway activity during HT1080 primary tumour cell invasion. Time-lapse video showing cWnt pathway activity in HT1080 TOP Venus reporterexpressing cancer cells during primary tumour cell invasion. Inset shows HT1080 cell intravasation into the blood vessel in the upper right part of the tumour mass. Green arrow points to an HT1080 cell undergoing cWntlow to cWnthigh transition. Red arrow points to an HT1080 cell undergoing cWnthigh to cWntlow transition. Maximum intensity (3D) projection, 20 min/frame, 29 h total duration; Red = tdTomato, Green = Venus; 10x objective; scale bars = 20 μm.

Intravital imaging of Wnt/β-catenin and ATF2-dependent signalling pathways during tumour cell invasion and metastasis

Konstantin Stoletov, Saray Sanchez, Irantzu Gorroño, Miriam Rabano, Maria d. M. Vivanco, Robert Kypta, and John D. Lewis

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260285