Movie 2.

Visualisation of canonical Wnt pathway activity during HT1080 cell intravasation. Representative time-lapse video showing cWnt pathway activity in HT1080 TOP Venus reporter cells during intravasation. White arrows point to an intravasating HT1080 cWnt^low cell that is entering the blood stream and rolling along the vascular wall. Maximum intensity (3D) projection, 5 min/frame, 70 min total duration; Red = tdTomato, Green = Venus, White = Lectin 647; 25x objective; scale bars = 50 μm.