Movie 2.

Visualisation of canonical Wnt pathway activity during HT1080 cell intravasation. Representative time-lapse video showing cWnt pathway activity in HT1080 TOP Venus reporter cells during intravasation. White arrows point to an intravasating HT1080 cWntlow cell that is entering the blood stream and rolling along the vascular wall. Maximum intensity (3D) projection, 5 min/frame, 70 min total duration; Red = tdTomato, Green = Venus, White = Lectin 647; 25x objective; scale bars = 50 μm.

Intravital imaging of Wnt/β-catenin and ATF2-dependent signalling pathways during tumour cell invasion and metastasis

Konstantin Stoletov, Saray Sanchez, Irantzu Gorroño, Miriam Rabano, Maria d. M. Vivanco, Robert Kypta, and John D. Lewis

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260285