Movie 3.

Visualisation of canonical Wnt pathway activity during metastatic lesion invasion. Time-lapse video showing cWnt pathway activity in HT1080 TOP Venus cells during metastatic lesion invasion. Green arrow points to an HT1080 cell undergoing cWnt^low to cWnt^high transition. Red arrow points to an HT1080 cell undergoing cWnt^high to cWnt^low transition. Maximum intensity (3D) projection, 20 min/frame, 33 h 20 min total duration; Red = tdTomato, Green = Venus; 10x objective; scale bars = 20 μm.