Movie 3.

Visualisation of canonical Wnt pathway activity during metastatic lesion invasion. Time-lapse video showing cWnt pathway activity in HT1080 TOP Venus cells during metastatic lesion invasion. Green arrow points to an HT1080 cell undergoing cWntlow to cWnthigh transition. Red arrow points to an HT1080 cell undergoing cWnthigh to cWntlow transition. Maximum intensity (3D) projection, 20 min/frame, 33 h 20 min total duration; Red = tdTomato, Green = Venus; 10x objective; scale bars = 20 μm.

Intravital imaging of Wnt/β-catenin and ATF2-dependent signalling pathways during tumour cell invasion and metastasis

Konstantin Stoletov, Saray Sanchez, Irantzu Gorroño, Miriam Rabano, Maria d. M. Vivanco, Robert Kypta, and John D. Lewis

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260285