Visualisation of Wnt/β-catenin and ATF2-dependent signalling activities during HTDR cell invasion within metastatic lesions. Time-lapse video showing Wnt/β-catenin-(red) and ATF2-dependent (green) reporter activities during HTDR cell metastatic lesion invasion. Maximum intensity (3D) projection, 20 min/frame, 33 h total duration; Red = mCherry, Green = GFP; 10x objective; scale bars = 50 μm.