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Movie 3.

MHB neuroepithelial cell GFP-CLC dynamics. Timelapse video of a 28 hpf zebrafish embryo transiently expressing GFP-CLC. Embryos were imaged from a coronal perspective with the orientation of neuroepithelial cells indicated by apical and basal labels. Alongside the multiple endocytic intermediates and Golgi / endosomal pools of GFP-CLC seen in the neuroepithelial tissue, GFP-CLC can also be seen localised to a mitotic spindle in a cell undergoing division at the apical surface.

Assessment of endocytic traffic and Ocrl function in the developing zebrafish neuroepithelium

Daniel M. Williams, Lale Gungordu, Anthony Jackson-Crawford, and Martin Lowe

J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.260339