Movie 1.

Single, dividing cell from an asynchronous population shows dynamic pHi changes during cell cycle progression. Video of a dividing cell in an asynchronous population over 24 h, compiled from a single Z-plane captured every 20 min. Arrows show cell of interest and reappear to show timing of prophase/cytokinesis and two daughter cells, scale bar: 20 μm. Ratiometric display of pHluorin/mCherry fluorescence ratios. Video is the same cell as Fig. 4A and ratiometric scale is identical.