Movie 3.

Single, dividing cell released from S phase synchronization shows dynamic pHi changes during cell cycle progression. Video of a dividing cell over 24 h after release from a double thymidine block, compiled from a single Z-plane captured every 20 min. Arrows show cell of interest and reappear to show timing of prophase/cytokinesis and two daughter cells, scale bar: 20 μm. Ratiometric display of pHluorin/mCherry fluorescence ratios. Video is the same cell as Fig. 5A and ratiometric scale is identical.

J Cell Sci ; doi: 10.1242/jcs.260458