Movie 2.

Amoeba viability under hypo-osmotic conditions. Time lapse video microscopy of Dictyostelium discoideum DH-1 after exposure to hypo-osmotic conditions as described, demonstrating that the amoebae were intact and viable as shown by movement, cellular integrity, and contractile vacuole action. Related to the Material and Methods section “Cells and Culture conditions”.

Endosomal vesicle fusion machinery is involved with the contractile vacuole in Dictyostelium discoideum

Paul T. Manna, Lael D. Barlow, Inmaculada Ramirez-Macias, Emily K. Herman, and Joel B. Dacks

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260477