To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 1. Dynamics of actin and VCC in HIV-1-infected macrophage MDMs transduced with Lifeact-mCherry were infected with HIV-1-GAG-iGFP-ΔENVVSVG (MOI=1.0). Time-lapse imaging was performed at day 6 after infection. Scale bar = 5 μm Release of HIV-1 particles from macrophages is promoted by an anchored cytoskeleton and driven by mechanical constraints Vasco Rodrigues, Sarah Taheraly, Mathieu Maurin, Mabel San-Roman, Emma Granier, Anaël Hanouna, and Philippe Benaroch J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.260511 Download Original Video