Movie 2.
Dynamics of the VCC after jasplakinolide treatment MDMs were grown in 24-well plates, infected with HIV-1-ΔENV-GAG-iGFPVSVG at a MOI=1.0 for 4 days and subsequently treated with DMSO or jasplakinolide (100 nM). Cells were then placed in an Incucyte S3 and phase contrast and GFP images were acquired at a 30 min frequency.
Release of HIV-1 particles from macrophages is promoted by an anchored cytoskeleton and driven by mechanical constraints
J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.260511