Dynamics of the VCC after PF396 treatment MDMs were grown in 24-well plates, infected with HIV-1-ΔENV-GAG-iGFPVSVG at a MOI=1.0 for 4 days and subsequently treated with DMSO or PF396 (1 μM). Cells were then placed in an Incucyte S3 and phase contrast and GFP images were acquired at a 2-hour frequency.