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Movie 6.

rAprA reduces motility of pipkinA-/pipkinA-GFP cells. pipkinA-/pipkinAOE cells were placed in wells in HL5 and were allowed to adhere for 30 minutes. rAprA was then added to 300 ng/ml, and cells were imaged starting 1 minute after adding rAprA. Elapsed time in minutes:seconds is at upper left. The horizontal field of view is 107 μm. Video is representative of 4 independent experiments.

A phosphatidylinositol phosphate kinase inhibits Ras activation and regulates chemorepulsion in Dictyostelium discoideum

Sara A. Kirolos, Chance E. Hatfield, Ryan J. Rahman, Kristen M. Consalvo, Nolan K. Dittenhauser, and Richard H. Gomer

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260541