Live imaging of Golgi reassembly in vimentin KO MEFs. Related to Fig S7B. Prior to the start of the movie, complete Golgi disassembly was induced in vimentin KO MEFs by treatment with 5 μg/ml BFA for 90 min, following by washing into fresh medium. Imaging started at X min post-washout and was captured on a 3i spinning disc confocal microscope. Scale bar, 10 μm.
Teresa Vitali, Rosa Sanchez-Alvarez, Tomasz M. Witkos, Ioannis Bantounas, Marie F. A. Cutiongco, Michal Dudek, Guanhua Yan, Alexander A. Mironov, Joe Swift, and Martin Lowe
J Cell Sci
2023. 136:None-None; doi: 10.1242/jcs.260577