Movie 2.
MEF cells complete nuclear transit without contracting the rear cortex. Representative time-lapse sequence of a MEF expressing mCherry2-Actin chromobody (magenta) and GFP-Nesprin-2 (cyan) transiting through a ≤2×5 μm2 constriction forming actin fibers that pull at the leading edge of the nucleus. Scale bar: 10 μm.
Rear cortex contraction aids in nuclear transit during confined migration by increasing pressure in the cell posterior
J Cell Sci 2024. 137:None-None; doi: 10.1242/jcs.260623