Movie 1.

ZnUMBA in EGTA treated zebrafish embryos. Time-lapse fluorescence movie of embryos labeled with membrane RFP in red to detect EVL and deep cells, FluoZin-3 in green, and Alexa Fluor 647-Dextran in magenta. Representative control embryos incubated in Danieau's medium (upper panel) and embryos incubated in Calcium free isotonic Ringer's solution with 10-20 mM EGTA (lower panel). Movie was started at 4 hpf. Scale bar, 10 μm. Time interval, 2 min.