Movie 3.

HUVEC-monolayer dataset corrected using Fast4DReg and Correct3DD. HUVEC cells labeled with SiR-actin were imaged in 3D using a Zeiss 880 Airyscan confocal microscope. This dataset suffered from significant drift, corrected using Fast4DReg and Correct3DD. The top panels show a selected z-plane of the uncorrected and corrected videos. The bottom panels show the y-projection of the uncorrected and corrected videos to visualize the axial drift. Scale bar 5 μm.