Movie 5.

The mouse lung dataset corrected using Fast4DReg. AsPC1 cancer cell migrating inside the mouse lung vasculature was imaged ex-vivo in 3D using a Zeiss 880 Airyscan confocal microscope. The original dataset suffered from lateral and axial drift, which was corrected using Fast4DReg. The top panels show z-projection of the uncorrected and corrected videos. The bottom panels show the y-projection of the uncorrected and corrected videos to visualize the axial drift. Scale bar 10 μm.

Fast4DReg – fast registration of 4D microscopy datasets

Joanna W. Pylvänäinen, Romain F. Laine, Bruno M. S. Saraiva, Sujan Ghimire, Gautier Follain, Ricardo Henriques, and Guillaume Jacquemet

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260728