Movie 1.

The WASp(CRIB)-mScarlet-I-WASp(CRIB) Cdc42 sensor relocating in a HeLa cell. Spinning disk time lapse movie of a HeLa cell co-expressing Lck-FRBmTurquoise2 (left), YFP-FKBP-ITSN1(DHPH) (middle) and WASp(CRIB)-mScarlet-IWASp(CRIB), stimulation with 100 nM rapamycin after 40 s. The time stamper represents min:s from the beginning of the recording.