Movie 2.

The mScarlet-I-1xWASp(CRIB) Cdc42 sensor relocating in a HeLa cell. Spinning disk microscopy images of a HeLa cell co-expressing Lck-FRBmTurquoise2 (not shown), YFP-FKBP-ITSN1(DHPH) (not shown) and mScarlet-I-1xWASp(CRIB), stimulated with 100 nM rapamycin at 1:40 min. The time stamper represents min:s from the beginning of the recording.