Movie 3.

The dimericTomato-1xWASp(CRIB) Cdc42 sensor relocation in a HeLa cell. Spinning disk microscopy images of a HeLa cell (not the cell depicted in Fig. 5B) co-expressing Lck-FRB-mTurquoise2 (not shown), YFP-FKBP-ITSN1(DHPH) (not shown) and dimericTomato-1xWASp(CRIB), stimulated with 100 nM rapamycin at 1:20 min. The time stamper represents min:s from the beginning of the recording.