Movie 4.
Co-imaging of the optimized Cdc42 and Rho relocation sensors. Spinning disk images of Hek 293T cells co-expressing Lck-FRB-ECFP(W66A)-black mutant (not shown), YFP-FKBP-ITSN1(DHPH) (top), Rho sensor mTurquoise2-3xrGBD (middle) and the Cdc42 sensor dimericTomato-WASp(CRIB) (bottom), first stimulated with 2 U/ml human α-thrombin, and then with 100 nM rapamycin. The time stamper represents min:s from the beginning of the recording.
J Cell Sci ; doi: 10.1242/jcs.260802