Movie 1.

Vacuolar killing of SPN. Time lapse fluorescence imaging demonstrating degradation of tRFP expressing SPN (red) following infection in A549s stably expressing YFP-Gal8 (green). Live cell imaging was performed under spinning disc confocal microscope starting at 60 min p.i. Total 33 z-stacks were collected, out of which MIP of stacks 4-17 were shown in the movie. The movie depicts kinetics of Gal8 association with SPN and its degradation while being associated with Gal8 signal. Time interval, 12 min; scale bar, 1.5 μm.