Movie 12.

Intracellular WT SPN containing vacuole is marked with Galectin3. Time lapse imaging of GFP-Ply expressing WT SPN in mStrawberry-Gal3 expressing A549s. Live cell imaging was performed under Laser scanning confocal microscope starting at 30 min p.i. The videos showcase the kinetics of association of Gal3 with PCVs containing WT SPN. Time interval, 15 min; scale bar, 5 μm.