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Movie 1.

FcRn-mCherry dynamics. BOECs were transduced with recombinant B2M-FcRn-mCherry lentivirus for 24 hours, monolayers washed and cells incubated for an additional 24 hours. Live BOECs were imaged at 37°C and 5% CO2 with a FV3000 Olympus confocal microscope. The delay between image capture was 5.84 sec.

The endosomal system of primary human vascular endothelial cells and albumin–FcRn trafficking

Andreas Pannek, Janine Becker-Gotot, Steven K. Dower, Anne M. Verhagen, and Paul A. Gleeson

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260912